chromium single cell 5 library gel bead kit (10X Genomics)
86
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10X Genomics
chromium single cell 5 library gel bead kit
Chromium Single Cell 5 Library Gel Bead Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chromium+single+cell+5+library+gel+bead+kit/cellranger/pmc13054087-49-21-30
Average 86 stars, based on 1 article reviews
Chromium Single Cell 5 Library Gel Bead Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chromium+single+cell+5+library+gel+bead+kit/cellranger/pmc13054087-49-21-30
Average 86 stars, based on 1 article reviews
chromium single cell 5 library gel bead kit - by Bioz Stars,
2026-10
86/100 stars
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Single Cell:Article Title: Systemic profiling of immune responses in healthy adults vaccinated with an RBD-targeting COVID-19 mRNA vaccine Article Snippet: .. Single-cell capture and library construction were performed using the Article Title: Effector memory-type regulatory T cells display phenotypic and functional instability. Article Snippet: Ex vivo FACSorted Treg cells and conventional CD4+ T cells were labeled with anti- human TotalSeq- C hashtag antibodies (BioLegend) allowing the pooling of samples, followed by labeling with antihuman TotalSeq- C antibodies (BioLegend) targeting a panel of extracellular proteins. .. Antibody and transcriptome libraries were prepared by using the Article Title: Single-cell profiling of acral melanoma infiltrating lymphocytes reveals a suppressive tumor microenvironment. Article Snippet: CD3+CD45+ cells were sorted and loaded on a Chromium instrument (10x Genomics). .. Preparation of scRNA and scTCR libraries Sample processing for scRNA- seq and scTCR- seq was performed using a Article Title: Immunological characterization and comparison of children with COVID-19 from their adult counterparts at single-cell resolution. Article Snippet: Single-cell 5′ mRNA sequencing and data preprocessing Suspended PBMCs of COVID-19 patients and healthy controls were loaded into Chromium microfluidic chips and barcoded with a 10X ChromiumController (10X Genomics). .. RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed with reagents from a Article Title: Effector memory–type regulatory T cells display phenotypic and functional instability Article Snippet: Ex vivo FACSorted T reg cells and conventional CD4 + T cells were labeled with anti-human TotalSeq-C hashtag antibodies (BioLegend) allowing the pooling of samples, followed by labeling with anti-human TotalSeq-C antibodies (BioLegend) targeting a panel of extracellular proteins. .. Antibody and transcriptome libraries were prepared by using the Article Title: Identification of tumor initiating cells and early marker genes in histologically normal colonic mucosa that lead to neoplastic transformation Article Snippet: Single-cell suspensions were generated from freshly collected colon tissues using the Neural Tissue Dissociation Kit (Miltenyi Biotec, #130-092-628) according to the manufacturer’s protocol. .. Following dissociation, single cells were encapsulated into nanodroplets using the Chromium Controller (10X Genomics), and single-cell libraries were constructed using the Article Title: A single-cell map of patients with non-small cell lung cancer harboring rare-driver mutations after anti-PD-1 treatment. Article Snippet: The effects of the tumor microenvironment the therapeutic efficacy of combining chemotherapy with checkpoint inhibitors in patients with lung cancer harboring rare -driver mutations remain unclear.. We utilized single-cell RNAand T-cell receptor (TCR) -sequencing to explore the immune and stromal cell profiles of 12 tumors and five tumor-adjacent tissues in seven patients with non-small cell lung cancer (NSCLCs) with rare -driver mutations treated with anti-PD-1 agents combined with chemotherapy.. A class of highly expanded T -cells, known as GZMK + CD8 effector memory T cells (GZMK + CD8+Tem), was enriched in both responsive tumors with and without rare driver mutations, suggesting similar anti-tumor immune mechanisms in both cohorts and that high levels of GZMK + CD8+Tem might be associated with effective responses to combination therapy. Article Title: Transcription of HIV-1 at sites of intact latent provirus integration Article Snippet: .. 10X Genomics gene expression and V(D)J libraries were generated with the Reverse Transcription:Article Title: Immunological characterization and comparison of children with COVID-19 from their adult counterparts at single-cell resolution. Article Snippet: Single-cell 5′ mRNA sequencing and data preprocessing Suspended PBMCs of COVID-19 patients and healthy controls were loaded into Chromium microfluidic chips and barcoded with a 10X ChromiumController (10X Genomics). .. RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed with reagents from a Sequencing:Article Title: Immunological characterization and comparison of children with COVID-19 from their adult counterparts at single-cell resolution. Article Snippet: Single-cell 5′ mRNA sequencing and data preprocessing Suspended PBMCs of COVID-19 patients and healthy controls were loaded into Chromium microfluidic chips and barcoded with a 10X ChromiumController (10X Genomics). .. RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed with reagents from a Construct:Article Title: Immunological characterization and comparison of children with COVID-19 from their adult counterparts at single-cell resolution. Article Snippet: Single-cell 5′ mRNA sequencing and data preprocessing Suspended PBMCs of COVID-19 patients and healthy controls were loaded into Chromium microfluidic chips and barcoded with a 10X ChromiumController (10X Genomics). .. RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed with reagents from a Article Title: Identification of tumor initiating cells and early marker genes in histologically normal colonic mucosa that lead to neoplastic transformation Article Snippet: Single-cell suspensions were generated from freshly collected colon tissues using the Neural Tissue Dissociation Kit (Miltenyi Biotec, #130-092-628) according to the manufacturer’s protocol. .. Following dissociation, single cells were encapsulated into nanodroplets using the Chromium Controller (10X Genomics), and single-cell libraries were constructed using the Concentration Assay:Article Title: A single-cell map of patients with non-small cell lung cancer harboring rare-driver mutations after anti-PD-1 treatment. Article Snippet: The effects of the tumor microenvironment the therapeutic efficacy of combining chemotherapy with checkpoint inhibitors in patients with lung cancer harboring rare -driver mutations remain unclear.. We utilized single-cell RNAand T-cell receptor (TCR) -sequencing to explore the immune and stromal cell profiles of 12 tumors and five tumor-adjacent tissues in seven patients with non-small cell lung cancer (NSCLCs) with rare -driver mutations treated with anti-PD-1 agents combined with chemotherapy.. A class of highly expanded T -cells, known as GZMK + CD8 effector memory T cells (GZMK + CD8+Tem), was enriched in both responsive tumors with and without rare driver mutations, suggesting similar anti-tumor immune mechanisms in both cohorts and that high levels of GZMK + CD8+Tem might be associated with effective responses to combination therapy. Gene Expression:Article Title: Transcription of HIV-1 at sites of intact latent provirus integration Article Snippet: .. 10X Genomics gene expression and V(D)J libraries were generated with the Generated:Article Title: Transcription of HIV-1 at sites of intact latent provirus integration Article Snippet: .. 10X Genomics gene expression and V(D)J libraries were generated with the |